Immobilized GalactEXO

795.001,295.00

Immobilized GalactEXO is an immobilized β-galactosidase for efficient galactose hydrolysis.

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Description

Immobilized GalactEXO contains spin columns with immobilized GalactEXO on agarose beads. The immobilized enzymes hydrolyzes β1-3 and β1-4 galactose on N- or O-glycosylated proteins or free oligosaccharides. Immobilized GalactEXO is available as a spin columns for hydrolysis of galactose from 0.5 mg glycoprotein.

Detailed Information

Product Group

Source/Origin

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Product Composition

Digestion Site

,

Specificity

Substrates

,

Reaction Conditions

Digestion Buffers

Buffer Compatibility

Unit Definition

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Application

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FAQs

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

No. Branching fucose linked to the same residue as the β-galactose inhibits GalactEXO activity and needs to be removed prior to GalactEXO treatment.

Yes, both beta1-3 linked galactose on O-glycans and beta1-4 galactose on N-glycans are hydrolyzed by GalactEXO.

For most substrates such as antibodies or Fc-fusion proteins a 2 h incubation results in hydrolysis of over 95% of the present galactose. More complex substrates with high degree of galactosylation may require longer incubation times.

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