Immobilized FabALACTICA

365.003,305.00

Immobilized FabALACTICA generates intact Fab and Fc fragments from human IgG1

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Description

Immobilized FabALACTICA contains FabALACTICA (IgdE) enzyme immobilized on agarose beads. The immobilized enzyme digests human IgG1 at one single amino acid position above the hinge generating intact Fc and Fab fragments. Immobilized FabALACTICA is available in spin columns for digestion of 0.5 – 100 mg of human IgG1 without residual enzyme in the final preparation.

Detailed Information

Application

Antibody Fragmentation, Antibody Characterization

Product Group

FabALACTICA

Source/Origin

Cloned from S. agalactiae, Produced in E. coli (His-tagged), 70 kDa

Product Composition

Immobilized on agarose beads (20% EtOH)

Digestion Site

Above Hinge

Specificity

KSCDKT / HTCPPCP (above the hinge)

Substrates

Human IgG1

Reaction Conditions

16-18 hour reaction at RT according to protocol specifications

Digestion Buffers

150 mM Na-phosphate (pH 7.0)

Buffer Compatibility

Optimal activity is obtained in 100-150 mM sodium phosphate buffers at pH 6.5 to 7.5. Up to 150 mM of NaCl can be added without affecting the enzymatic activity.

Unit Definition

0.1 ml settled resin digests ≥ 95% of 0.5 mg Human IgG1

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