IgGZERO LE

530.00

IgGZERO LE (Low Endotoxin) deglycosylates biantennary Fc-glycans on IgG

530.00
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Description

IgGZERO LE (EndoS) is an IgG-specific endoglycosidase that rapidly deglycosylates biantennary Fc glycans after the core GlcNAc on IgG from several species and subclasses. IgGZERO LE contains low endotoxin levels <0.4 EU/vial and is available as lyophilized powder for deglycosylation of 1-5 mg of IgG.

Detailed Information

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FAQs

First you could increase incubation time and increase temperature to 37°C.

Another explanation for not complete deglycosylation could be if the antibody has higher amounts of high-mannose, some hybrid glycans or bisected glycans. For these glycans IgGZERO has low or no activity. Especially, high-mannose glycans are particular resistant to digestion.

If longer incubation time and increased temperature does not increase deglycsylation we would recommend you to try our other endoglycosidase GlycINATOR (EndoS2). This enzyme has much higher activity on these types of glycans sometimes seen in m.Abs.

Yes, many buffers are compatible with IgGZERO/DeGlycIT. Please keep pH close to neutral.

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