GlycOCATCH

625.00

GlycOCATCH purifies and enriches mucin-type O-glycosylated proteins and peptides

625.00
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Description

GlycOCATCH contains spin columns with inactive OpeRATOR (OgpA) enzyme immobilized on agarose beads. The immobilized inactive enzyme specifically binds core 1 O-glycans for enrichment and purification of mucin-type O-glycosylated proteins and peptides. GlycOCATCH performance is optimal after desialylation using SialEXO and captured O-glycoproteins can be eluted using 8 M urea or active OpeRATOR enzyme. GlycOCATCH is available in spin columns for affinity-capture of 200 ug of O-glycoprotein and supplied with lyophilized SialEXO for preparative desialylation and OpeRATOR enzyme for elution.

Detailed Information

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FAQs

Before you load your sample, make sure that the protease in the solution is inactivated, and that the pH, buffer, chaotrope and NaCl concentrations are within the recommended ranges (according to the instructions).

No, when the glycoprotein is digested with OpeRATOR, the binding ability of GlycOCATCH is lost.

The OpeRATOR enzyme digests O-glycosylated proteins N-terminally of the serine (S) and threonine (T) glycosylation sites. If you want to know at which S or T a specific O-glycan is sited, we recommend elution with OpeRATOR. We also recommend elution with OpeRATOR if you have a glycoprotein with suitable distances between the O-glycan sites and suitable distances between the O-glycans and the N/C-terminals, and you want to generate peptides of suitable size for your purpose.

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