Name: GlyCLICK AlexaFluor647
SKU: 10595
Price: 925.00 EUR
Availability: InStock

Description

GlyCLICK AlexaFluor647 is a kit that contains all reagents needed for site-specific conjugation of IgG using AlexaFluor^®647. Spin columns with GlycINATOR (EndoS2) enzyme immobilized on agarose beads deglycosylates IgG from several species and subclasses by hydrolyzing any Fc glycoform after the inner most GlcNAc. The GalT enzyme supplied in solution enables incorporation of the UDP-GalNAz regent to the exposed GlcNAc sugar for subsequent click chemistry incorporating the AlexaFluor^®647 label. GlyCLICK AlexaFluor647 is available in kit formats to facilitate labeling of 250 ug or 2 mg of IgG.

Detailed Information

Application	Antibody Conjugation, Immunofluoresence Microscopy, Flow Cytometry
Product Group	GlyCLICK
Source/Origin	Cloned from S. pyogenes, Produced in E. coli (His-tagged), Engineered b-1,4-galactosyltransferase (Y289L)
Product Composition	Enzymatic components: Immobilized GlycINATOR (EndoS2) on agarose beads (20% EtOH), beta-1,4-galactosyltransferase (GalT)
Digestion Site	IgG Fc-glycans
Specificity	GlycINATOR hydrolyzes Beta-1,4-linked GlcNAc residues on all IgG Fc-glycoforms for site-specific azide activation using GalT and complete labeling using SPAAC
Substrates	Human IgG1, Human IgG2, Human IgG3, Human IgG4, Cow IgG, Fc-fusion proteins, Goat IgG, Horse IgG, Monkey IgG, Mouse IgG, Rabbit IgG, Rat IgG, Sheep IgG
Reaction Conditions	Deglycosylation (GlycINATOR): 30 min incubation at 37°C, Azide Activation (GalT): O/N incubation at 30°C, Labeling (SPAAC): O/N incubation at 25°C, *The protocol can vary depending on kit size/amount of IgG
Digestion Buffers	TBS (pH 7.4)
Buffer Compatibility	GlyCLICK steps are performed in TBS buffer, provided in the kit., For purification of azide activated Ab, TBS or PBS can be used., Sodium azide interferes with the click reaction and must be avoided., Avoid phophate buffers, not compatible with buffer additive (precipitation).
Additives/Co-factors	GalT requires buffer additive for activity., Buffer additive is included in the GlyCLICK kit.
Inhibitors	Denatured IgG
Unit Definition	Components for antibody conjugation of up to 0.25-2 mg IgG
Label	AlexaFluor 647

GlyCLICK Alexa Fluor 647 250ug quantity	GlyCLICK Alexa Fluor 647 250ug (L1-F03-025)	€1,010.00
GlyCLICK Alexa Fluor 647 2mg quantity	GlyCLICK Alexa Fluor 647 2mg (L1-F03-200)	€1,870.00

GlyCLICK AlexaFluor647

Description

Detailed Information

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