GlycINATOR

450.00

GlycINATOR rapidly deglycosylates all Fc-glycans on IgG

450.00
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Description

GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that rapidly deglycosylates IgG Fc glycans after the core GlcNAc. The GlycINATOR enzyme hydrolyzes all Fc glycoforms on IgG from several species and subclasses. GlycINATOR is available as lyophilized powder for deglycosylation of 2 mg of IgG. GlycINATOR LE contains low endotoxin levels <0.4 EU/vial.

Detailed Information

Application

Antibody Deglycosylation, Afucosylation Assessment, Immunoassays

Product Group

GlycINATOR

Source/Origin

Cloned from S. pyogenes, Produced in E. coli (His-tagged), 92 kDa

Product Composition

Lyophilized in TBS, Purified to ≥ 95% homogeneity

Digestion Site

IgG Fc-glycans

Specificity

Hydrolyzes Beta-1,4-linked GlcNAc residues on all IgG Fc-glycoforms

Substrates

Human IgG1, Human IgG2, Human IgG3, Human IgG4, Cow IgG, Fc-fusion proteins, Goat IgG, Horse IgG, Monkey IgG, Mouse IgG, Rabbit IgG, Rat IgG, Sheep IgG

Reaction Conditions

30 minute reaction at 37°C according to protocol specifications

Digestion Buffers

PBS (pH 7.4), TBS (pH 7.4)

Buffer Compatibility

Optimal activity at physiological conditions. Other buffers or pH (6.0 – 8.0) can be used but the reaction conditions need to be optimized.

Inhibitors

Denaturated IgG

Unit Definition

1 unit deglycosylates ≥ 95% of 1 µg Human IgG1 Fc-glycans

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