€470.00
GingisKHAN digests human IgG1 at a single site above the hinge under mild reducing conditions
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 and Fc-fusion proteins carrying an IgG1 hinge at one single amino acid position above the hinge generating intact Fc and Fab fragments. GingisKHAN is available as lyophilized powder for digestion of 2 mg of human IgG1 and includes GingisKHAN Reducing Agent (lyophilized cysteine) to facilitate mild reducing conditions.
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| Digestion Site | |
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| Reaction Conditions | 1 hour reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
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| Additives/Co-factors | Requires Reducing Conditions, 2 mM Cysteine (GingisKHAN Reducing Agent (10X) included) |
| Inhibitors | |
| Unit Definition |
Genovis provides a GingisKHAN Fab kit containing a specific Capture Select CH1 resin for further purification of the Fab. See this page for more information.
The enzyme is a cysteine protease that can be inhibited by alkylating agents such as iodoacetamide. There are also several other known inhibitors to this enzyme, like KYT-36.
The ready-to-use reaction buffer additive has been optimized to provide full enzymatic activity without affecting the disulphide bridges between the light chain and Fd region of the heavy chain, keeping the Fab intact. We recommend preparing a fresh solution of pH neutral cysteine, for use the same day, with a final reaction concentration of 2mM.
No, GingisKHAN is a general protease. However, under the recommended reaction conditions, it digests native human IgG1 at one specific site above the hinge.
