FragIT Z Kit

400.001,070.00

FragIT Z kit easily generates and purifies F(ab’)2 and Fc/2 fragments from mouse IgG2a and IgG3

400.00
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Description

FragIT Z kit contains FragIT Z spin columns with FabRICATOR Z (IdeZ) enzyme immobilized on agarose beads and CaptureSelectTM spin columns for affinity capture of Fc fragments. FragIT digests antibodies or Fc-fusion proteins at one single amino acid position below the hinge generating Fc/2 and F(ab’)2 fragments. CaptureSelectTM spin columns capture Fc fragments for purification of F(ab’)2 and Fc fragments. FragIT Z kit is available in spin column format for digestion and purification of 0.5 – 2.5 mg of mouse IgG2a and IgG3 without residual enzyme in the final preparation.

Detailed Information

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FAQs

We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.

It is possible to elute the Fc fragments from the CaptureSelect column using a low pH buffer, for example a 0.1M Glycine buffer pH=3.0. The pH must immediately after elution be adjusted to neutral pH by adding 0.1x elution volume of 1M Tris pH=8.0.

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