FabRICATOR-HPLC offers on-column digestion of mAbs for automated subunit generation



FabRICATOR-HPLC contains the FabRICATOR (IdeS) enzyme immobilized on HPLC compatible resin for robust on-column digestion in automated workflows. FabRICATOR-HPLC provides digestion of human IgG and Fc-fusion proteins at one single amino acid position below the hinge generating Fc and F(ab’)2 fragments.

Detailed Information



Product Group


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Product Composition

Digestion Site



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Reaction Conditions


Digestion Buffers

Buffer Compatibility


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Unit Definition


We have tested concentrations as low 0.025 mg/ml without any measurable effect on digestion efficiency. However, low concentration samples require a longer injection time and consequently lead to a broader elution peak from the FabRICATOR-HPLC column.

Yes, it is possible to digest the antibody in the first dimension and then separate the subunit in the second dimension. See application note

The FabRICATOR-HPLC can run >400 samples of 10 ug of IgG.

FabRICATOR-HPLC is an HPLC column with the FabRICATOR (IdeS) protease immobilized on a HPLC compatible matrix for automated antibody subunit generation.

We have tested PBS and 150 mM ammonium acetate, pH7 with good results. More buffers were found to be compatible with FabRICATOR digestion in solution (more info) and should be suitable for FabRICATOR-HPLC as well. However, the ionic strength of the buffer influences retention of the antibody fragments on the FabRICATOR-HPLC column and a minimum of 100-150 mM salt is necessary for IgG1 subunits to elute as a single, narrow peak. Other IgG subclasses or fusion proteins might need some optimization of buffer composition.

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