FabRICATOR-HPLC

1,675.00

FabRICATOR-HPLC offers on-column digestion of mAbs for automated subunit generation

1,675.00
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Description

FabRICATOR-HPLC contains the FabRICATOR (IdeS) enzyme immobilized on HPLC compatible resin for robust on-column digestion in automated workflows. FabRICATOR-HPLC provides digestion of human IgG and Fc-fusion proteins at one single amino acid position below the hinge generating Fc and F(ab’)2 fragments.

Detailed Information

Application

Antibody Fragmentation, Automated Antibody Fragmentation

Product Group

FabRICATOR

Source/Origin

Cloned from S. pyogenes, Produced in E. coli (His-tagged), 37.7 kDa

Product Composition

Immobilized on HPLC compatible resin packed in PEEK column 2.1 x 50mm in PBS, pH 7.4 with 0.02% sodium azide.

Digestion Site

Below Hinge

Specificity

CPAPELLG / GPSVF (below the hinge)

Substrates

Human IgG1, Human IgG2, Human IgG4, Monkey IgG, Rabbit IgG, Rat IgG, Sheep IgG

Reaction Conditions

Operating Conditions: Operating pH 6.5 – 8.0, Operating Temperature 37° C, Maximal Pressure 100 bar, Typical flow rate 0.025-0.05 mL/min., Column Performance: Number of days of continuous operation: >10*, Injections per column: >200*. * Depending on specific application.

Digestion Buffers

PBS (pH 7.4)

Buffer Compatibility

150 mM ammonium acetate, pH 7 (ionic stregth of >150 mM is necessary). Optimization may be required.

Inhibitors

Iodoacetate 20 mM, Iodoacetamide 1 mM, pH < 5.2

Unit Definition

≥ 95% digestion of 10 μg hIgG1 in less than 5 minutes residence time at 37°C as monitored by RP-LC

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