Articles by Maria Ekemohn

NEW! Hydrolysis of Flexible Linkers using GlySERIAS

June 14, 2022 | New Product, News |

GlySERIAS is a unique enzyme that digests flexible glycine-rich fusion protein linkers such as Gly4Ser and GlyxSery (GS) and polyglycine (G) linkers.
 
The enzyme enables separation of the individual domains of multi-functional fusion proteins to facilitate characterization and increase the understanding of these molecules. Middle-level analysis of fusion proteins serves to both reduce the overall sample complexity and allow for domain-specific identification and monitoring of post-translational modifications.
 
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NEW! Sugar-free Proteins in a Few Hours using OmniGLYZOR

June 6, 2022 | New Product, News |

OmniGLYZOR hydrolyzes N- and mucin-type O-glycans. It contains a mixture of immobilized enzymes for fast and efficient removal of N- and simple mucin-type O-glycans on antibodies, fusion proteins and other glycosylated proteins.

Removal of glycans is widely used to reduce heterogeneity to facilitate analysis of the protein by for example mass spectrometry. Deglycosylation can also be used to study the functional role of the glycans.
 
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SmartEnzymes™ for Gene Therapy

May 11, 2022 | News, Products |

The development of advanced gene therapies is promising and gives thousands of patients new hope for a curative treatment. However, many of the current gene therapies are using adeno-associated viruses as transporters of new genetic material. Up to 60% of patients may have antibodies against this viral vehicle and this is usually an exclusion criteria for being eligible for gene therapy. To overcome the presence of neutralizing antibodies, a new strategy includes the use of highly specific IgG proteases to digest the antibody population and increase the uptake of the new genetic material.
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NEW! Azide Activation of Human IgG using TransGLYCIT Azide Activation

April 4, 2022 | New Product, News |

We’re happy and proud to announce the launch of TransGLYCIT Azide Activation!
 
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SialEXO® 23 in a Study of Osteoclast Fusion

March 16, 2022 | References |

In a recent paper from Johns Hopkins University School of Medicine in Baltimore, removal of sialic acids from toll-like receptor 2 using SialEXO 23 was found to inhibit osteoclast fusion. These new findings in osteoclast recognition signaling are important for understanding the pathogenesis of different skeletal disorders, also showcasing the versatility of Genovis SmartEnzymes!
 
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FabRICATOR® in an Inline Electrochemical Reduction Workflow

February 17, 2022 | References |

An inline electrochemical reduction workflow for antibodies coupled with LC-MS analysis is described in this paper from the National Institute for Bioprocessing Research and Training in Dublin. The FabRICATOR enzyme (IdeS) is a key component in the subunit analysis by digesting antibodies at a single site below the hinge, generating a homogenous pool of F(ab’)2 and Fc subunits.
 
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SialEXO® in a Glycoform Analysis of Intact Erythropoietin

January 19, 2022 | References |

In this study, scientists at Leiden University Medical Center present a MALDI FT-ICR MS method for the glycosylation profiling of intact erythropoietin (EPO). The sialidase mix SialEXO significantly reduced the mass spectrum complexity and facilitated the interpretation of the complex spectra of intact EPO.
 
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GlycINATOR® in Peptide Mapping of Fc Domain-Drug Conjugates


The field of antibody-drug conjugates (ADCs) is rapidly emerging as a new, important class of cancer therapeutics. ADCs consist of monoclonal antibodies (mAbs) covalently linked to cytotoxic drugs, and after selective surface receptor binding on cancer cells, the drug is delivered into the intracellular compartment and can exert its cytotoxic effect.
 
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NEW! PNGase F for Hydrolysis of N-glycans

November 29, 2021 | New Product, News |

We are happy to announce that PNGase F for in-solution hydrolysis of N-glycans from glycoproteins is added to our portfolio of SmartEnzymes!
 
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NEW FabDELLO – The SmartEnzyme for Mutated IgG

November 18, 2021 | New Product, News |

We introduce FabDELLO, a protease that specifically digests human IgG1 at a single site above the hinge, generating intact Fab and Fc fragments within two hours with no need for reducing conditions. The enzyme is active on antibodies with mutated hinge regions and enables middle-level LC-MS for characterization of critical quality attributes.
 
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