With NIMT®FeOfection|PURPLE superior knockdown and low cell cytotoxicity is attained with high consistency and reproducibility from time-to-time and lot-to-lot.
786-O (human renal cell adenocarcinoma) transfected with NIMT®FeOfection|PURPLE at the same concentration using five different batches at five separate transfection events yields consistent more than 95% knockdown while maintaining high cell viability. The knockdown of Gapdh mRNA was measured with real time PCR and the cell viability with CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega). |