FragIT spin columns

FragIT microspin columns allow for easy and quick fragmentation of IgG into F(ab’)2 and Fc fragment. FragIT microspin columns come prefilled with FabRICATOR® enzyme immobilized on agarose. Fragmentation of IgG is typically associated with low yield, heterogeneity of produced fragments and a cumbersome and time-consuming process. All these shortcomings can now be solved using FragIT microspin columns for fragmentation of up to 0.5 mg IgG in less than 30 minutes.

Step	Description
	Remove lid. Centrifuge at 200 x g for 1 minute to remove preservative liquid. Add 100 uL of solution containing your IgG.
	Cap the spin column and incubate for 15 minutes at room temperature.
	Place the spin column in a microcentrifuge tube and remove lid. Collect the solution containing your antibodies by centrifugation at 1000 x g for 1 minute.

SDS page of human monoclonal IgG (Herceptin®) fragmented to F(ab')2 and Fc using FragIT microspin column.