FragIT Kit
The FragIT kit is the most convenient and economical way to produce pure F(ab')2 or Fab' from IgG. The FragIT kit consists of two spin columns one for the actual fragmentation and a second spin column to collect the Fc-fragments. This solution offers many advantges over existing technologies:
- Speed - the time for completion is very fast, usually less than one hour even for first-time users.
- Yield - usually more than 95% of starting material is recovered.
- Purity - the included affinity separation column binds selectively to the Fc fragment leaving very pure F(ab')2 fragments in the flow-through.
- Immuno reactivity - gentle handling of the fragments throughout the procedure minimizes reduction of immunoreactivity.
- Cost - highly cost-effective method due to high yield and the excellent quality of the fragments generated. The short handling time increases the value of this fragmentation solution for the end user.
IgG Species compatible with FragIT Kit
- Human IgG
- Humanized IgG
- Chimeric IgG
- Rabbit IgG
- Sheep IgG
- Monkey
- Mouse IgG2a
View example of FragIT-Kit using different speices of IgG
Mouse IgG2a requires a separate protocol. Contact Genovis for more information.
