deGlycIT spin columns

deGlycIT microspin columns allow for easy and quick deglycosylation of IgG. deGlycIT microspin columns comes prefilled with IgGZERO® enzyme immobilized on agarose. deGlycIT microspin columns are designed for deglycosylation of up to 0.5 mg IgG in less than 30 minutes.

Step	Description
	Remove lid. Centrifuge 200xg for 1 minute to remove the preservative liquid. Add 100 uL of solution containing your IgG.
	Cap the spin column and incubate for 15 minutes at room temperature
	Put the spin column in a microcentrifuge tube and remove the lid. Collect the solution containing your antibodies by centrifugation 1000xg for 1 minute.

Results and compatibility of deGlycIT spin column

SDS page (reducing condition) of human monoclona IgG (Herceptin®) before and after deglycosylation using deGlycIT microspin column.