In Analytical Biochemistry Chevreaux and co-workers describes the use of our FabRICATOR enzyme as an excellent tool to simplify mass spectrometry of monoclonal antibodies. By using the FabRICATORS’s very precise fragmentation capability the antibodies can be divided into three distinct sub-parts in very short time. The nice thing about this approach is that all the sub-parts are below 30 kD thereby reducing the complexity of antibodies when analyzed by mass spectrometry. The authors point out that glycoanalysis can be performed with high accuracy e.g. the extent of galactosylation and fucosylation.
The authors also speculate that using this method in combination with new highly resolutive MS tools will facilitate rapid investigation of the whole content of Mabs modification in one single experiment.
The paper entitled “Fast analysis of recombinant monoclonal antibodies using IdeS proteolytic digestion and electrospray mass spectrometry” can be found at http://www.ncbi.nlm.nih.gov/pubmed/21596014
