DNA Transfection

NIMT^®FeOfection is a transfection agent based on superparamagnetic nanoparticles with an iron oxide core. The particle surface is designed to promote non-covalent binding of DNA and facilitate effective transport of the resulting particle/DNA complexes into cells.

NIMT^®FeOfection is formulated to achieve consistently high transfection rates with markedly reduced toxicity. Using FeOfection, we have successfully transfected both standard screening cells such as HEK293 as well as more difficult-to-transfect cells such as K562.

NIMT^®FeOfection transfection agents offer several features that will simplify your transfection:

• Reduce Cell Toxicity – More stable and less toxic than leading transfection reagents
• Decrease steps and handling – Simply mix particles and DNA, incubate at room temperature and then add to cells
• Additional Benefits – The magnetically labelled cells can be seen with MRI

Different cell types have different needs for optimal uptake of macromolecules, such as transfection complexes. NIMT^®FeOfection is designed to be optimal for those needs. We can now offer different types of transfection agents that have been optimized for transfection of different cell types.

NIMT^®FeOfection|YELLOW + NIMT^®Booster is designed for transfection of suspension cells lines such as K562 and Jurkat cells. It has been shown that NIMT^®FeOfection|YELLOW + NIMT^®Booster also transfects adherent cell lines such as HEK293 successfully. NIMT^®FeOfection|PINK is designed for transfection of adherent cells such as COS-7. NIMT^®Booster can be added to NIMT^®FeOfection|PINK during the complex formation with DNA. The addition of NIMT^®Booster has been shown to improve the transfection efficiency several times on some adherent cells (e.g. U-2 OS). With NIMT^®FeOfection|TESTKIT you can try out which transfection reagent that suits your application or cell line the best.

Reduced Cell Toxicity

The stable nanoparticles have proven to be less toxic than other commercially available transfection agents. NIMT^®FeOfection transfection agents give high transfection rates and are at the same time mild and safe to use.

	NIMT®FeOfection|YELLOW + NIMT®Booster was compared to other commercially available transfection agents on suspension cell line K562. Results show that NIMT®FeOfection|YELLOW + NIMT®Booster yields high transfection rates together with low toxicity.
	On adherent cell lines NIMT®Booster has shown to improve transfection rates without affecting the toxicity. NIMT®FeOfection|YELLOW and NIMT®FeOfection|PINK with and without NIMT®Booster was compared to another leading commercially available transfection agent. When using NIMT®FeOfection products the toxicity becomes much lower than with the other commercially available agent and NIMT®Booster raises the transfection efficiency without affecting the proliferation.

Decrease Steps and Handling

NIMT^®FeOfection transfection agents are easy and fast to use. Simply mix particles (and NIMT^®Booster) with DNA, incubate 15-20 min in room temperature and then add to cells. Incubate the cells 24-48 hrs and then read transfection efficiency. NIMT^®FeOfection can be used with both forward and reverse transfection.

NIMT®FeOfection transfection agents are easy and fast to use. Simply mix particles (and NIMT®Booster) with DNA, incubate 15-20 min in room temperature and then add to cells. Incubate the cells 24-48 hrs and then read transfection efficiency. NIMT®FeOfection can be used with both forward and reverse transfection.

Additional Benifits

The superparamagnetic nanoparticles can be seen with magnetic resonance imaging (MRI). When cells are transfected with NIMT®FeOfection products they become magnetically labelled and can be tracked with MRI. Learn more about imaging and tracking here.