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Questions |
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Answers |
| Q1 |
Using a magnet, is it possible to separate transfected cells from untransfected cells? |
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A1 |
No. More than 95% of the cells take up the transfection particles and som of them may not be transfected. If you try to sort cells with a magnetic column all cells that have taken up the particles will be sorted. |
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| Q2 |
Is NIMT®FeOfection|PURPLE working on my cell line? |
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A2 |
In our cell line list you find all cells that have been tested with the NIMT®FeOfection products. |
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| Q3 |
Is NIMT®FeOfection|PURPLE toxic to cells? |
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A3 |
If you optimize the transfection according to protocol, NIMT®FeOfection|PURPLE is not toxic. |
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| Q4 |
I would like to transfect cells ex vivo and then transplant cells into an animal. Is it possible to track cells with MRI? |
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A4 |
Yes. All of the NIMT®FeOfection products are based on superparamagnetic nano particles and are tracable with the use of MRI. |
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| Q5 |
Is it possible to dilute NIMT®FeOfection|PURPLE in serum free medium, growth medium or buffers instead of water? |
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A5 |
No. To achieve optimal transfection efficiencies and avoid toxic flocculations of the particles, you should always use double distilled water in the dilution of particles and siRNA. |
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| Q6 |
Is it possible to store and reuse NIMT®FeOfection|PURPLE particles? |
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A6 |
No. NIMT®FeOfection|PURPLE should always be diluted prior to use. |
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| Q7 |
When I add the transfection complexes to the cells, the medium becomes diluted with water. How does that effect the cells? |
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A7 |
The cell proliferation can be affected negatively to a small degree. |
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| Q8 |
Are the any cell specific transfection protocols? |
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A8 |
No. To get the best transfection efficiencies it's best to do an optimization where you use your own cells, plate formate and type of siRNA. The transfection protocol include optimization protocol for adherent and suspension cell lines. Genovis is always willing to help you if you have questions about the optimization protocol, just send an e-mail to support and we will help you as much as we can. |
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| Q9 |
I want to transfect siRNA into suspenions cells, what product can I use? |
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A9 |
NIMT®FeOfection|PURPLE |
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| Q10 |
I want to transfect siRNA into adherent cell, what product can I use? |
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A10 |
NIMT®FeOfection|PURPLE |
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| Q11 |
What is the lowest siRNA concentration that can be used with NIMT®FeOfection|PURPLE? |
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A11 |
The lowest concentration that can be used depends on the cell line used. If it is a hard to transfect cell a higher siRNA concentration must be used (30-50 nM). An easy to transfect cell may only need 1-5 nM siRNA. |
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| Q12 |
After how long incubation time can knockdown be seen in cells? |
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A12 |
Full knockdown can be measured as early as 8 hours after transfection. |
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| Q13 |
Is it possible to co-transfect DNA and siRNA with NIMT®FeOfection transfection reagets? |
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A13 |
We would recommend you to first transfect your cells with DNA using NIMT®FeOfection|YELLOW and NIMT®Booster. Incubate cells 6 hours, change medium and then transfect then cells with siRNA using NIMT®FeOfection|PURPLE. |
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