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Questions |
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Answers |
Q1 |
Why has NIMT®FeOfection|PINK been discontinued? |
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A1 |
It have been shown that NIMT®FeOfection|YELLOW + NIMT®Booster is as, or more, effective transfection reagent for adherent cell lines than NIMT®FeOfection|PINK is. |
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| Q2 |
What product can be used for transfection of DNA into suspension cells? |
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A2 |
Use NIMT®FeOfection|YELLOW + NIMT®Booster when transfecting suspension cells with DNA. |
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| Q3 |
What product can be used for transfection of DNA into adherent cells? |
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A3 |
Use NIMT®FeOfection|YELLOW + NIMT®Booster when transfecting adherent cells with DNA. |
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| Q4 |
Is it possible to separate transfected cells from untransfected by using a magnet? |
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A4 |
No. More than 95% of the cells take up the transfection particles and some of them may not be transfected with DNA. If you then try to sort cells with a magnetic column all cells that have taken up the particles will become sorted. |
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| Q5 |
Is your transfection reagnet working on my cell line? |
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A5 |
In our cell line list you can find all cells that we know have been tested with the NIMT®FeOfection products. |
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| Q6 |
Is NIMT®FeOfection|YELLOW toxic to cells? |
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A6 |
If you optimize the transfection according to protocol, NIMT®FeOfection products are not toxic. |
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| Q7 |
I would like to transfect cells with NIMT®FeOfection|YELLOW + NIMT®Booster ex vivo and transplant cells into an animal. Is it possible to track cells with MRI? |
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A7 |
Yes. All NIMT®FeOfection products are based on superparamagnetic nano particles and are therefore traceable with MRI. |
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| Q8 |
Is it possible to dilute the NIMT®FeOfection|YELLOW in serum free media, growth medium or buffers instead of water? |
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A8 |
No. To achieve optimal transfection efficiencies and avoid toxic flocculations of the particles you should always use double distilled water when you dilute the particles and DNA prior to transfection. |
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| Q9 |
Is it possible to store and reuse diluted NIMT®FeOfection|YELLOW particles? |
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A9 |
No. The transfection particles should always be diluted prior to use. |
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| Q10 |
When you add the transfection complexes to cells, the medium becomes diluted with water. How does that affect the cells? |
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A10 |
The cell proliferation may become effected to a small degree. If desired, the medium can be changed 6 hours after transfection but the transfection rate may become little lower. |
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| Q11 |
Are there any cell specific transfection protocols? |
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A11 |
No. To get the best transfection efficiencies it's better to do an optimization where you use your own cells, plate formate and type of DNA. All instructions include an optimization protocol for adherent and suspension cell lines. Genovis support is always willing to help you do an optimization protocol, just send an e-mail to support with your specific transfection conditions. |
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| Q12 |
Is it possible to co-transfect DNA plasmids with NIMT®FeOfection transfection reagets? |
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A12 |
Yes, just mix your two DNA to one solution and add to the particles and Booster according to the normal protocol. |
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| Q13 |
Is it possible to co-transfect DNA and siRNA with NIMT®FeOfection transfection reagets? |
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A13 |
We would recommend you to first transfect your cells with DNA using NIMT®FeOfection|YELLOW and NIMT®Booster. Incubate the cells for approximately 6 hours, change medium and then transfect cells with siRNA using NIMT®FeOfection|PURPLE. |
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