RNAi

RNA interference (RNAi) is a powerful and widely used technology in elucidating gene function. A target gene is silenced in a sequence specific manner and the loss of that gene function is studied. Sequence specific dsRNA, delivered to the cell either as siRNA (short interfering RNA) or shRNA (short hairpin RNA), enters the RNAi pathway and complementary mRNA is destroyed.

Successful gene knock down depends on the potency of the siRNA and the efficiency of the delivery method. Genovis has developed a new transfection reagent for siRNA delivery featuring consistent high transfection efficiency and very low cell cytotoxicity.

NIMT^®FeOfection Transfection Reagents Offers:

• Excellent silencing at low siRNA concentrations – Minimizing non-specific and off target effects in the cell.
• Very Low Cell Toxicity - A stable nanoparticle with a solid iron oxide core significantly reduces cell toxicity.
• Easy and quick handling.
• Versatile – Gene knockdown in a broad range of cell lines

High gene knockdown at low siRNA concentrations is achieved with stable, uniform and size controlled transfection complexes. With a solid iron oxide core NIMT^®FeOfection transfection reagents provide a stable transfection carrier. To facilitate siRNA binding and promote cellular uptake are the iron oxide cores of NIMT^®FeOfection|PURPLE coated with a specific formulation of cationic and neutral lipids. 

With NIMT^®FeOfection transfection reagents effective gene silencing is reached in a wide range of cell lines including suspension cells, mesenchymal stem cells as well as adherent standard cell lines. 

Easy to Use Easy and quick handling - Simply mix NIMT®FeOfection|PURPLE with siRNA, incubate and add to cells.		Click on image to read more
Lipids Stay on the Particles Lipids that stay on the particles gives less impact on cell membranes, compared to e.g. lipid reagents.		Click on image to read more
Transfect with Low siRNA Concentration Lowering the siRNA concentration in the cell will reduce off-target effects. With an efficient transfection reagent the siRNA concnetration can be significantly lowered, preserving high gene knockdown with minimal off-target effects.		Click on image to read more
High Knockdown in a Broad Range of Cell Lines With NIMT®FeOfection|PURPLE it is possible to get efficient gene silencing toghether with low toxicity.		Click on image to read more
Exceedingly Low Toxicity NIMT®FeOfection transfection reagents imposes very low cytotoxicity. A solid iron oxide core provides a stable nano-carrier with minimal impact on cell viability.		Click on image to read more
Read knockdown as Early as 4 Hours Post Transfection Nanoparticles are readily taken up by cells and in certain cell lines significant knockdown is established within a few hours.		Click on image to read more
Stable Cores Protects siRNA The stable nano structures protects siRNA against degradation and results in prolonged gene silencing.		Click on image to read more
Highly Efficient Gene Silencing -  Applicable in RNAi High Throughput Screening. A long incubation time of transfection complexes, up to 3 hours, does not affect transfection efficiency. Lipid coated solid nanoparticles provide stable transfection complexes that do not grow in size over time.		Click on image to read more
Stable Nanocarriers - Highly Uniform Iron Oxide Particles with Lipid Coatings. NIMT®FeOfection|PURPLE consists of highly uniform nanoparticles with a narrow size distribution. A solid core of iron oxide is coated with specific lipids to form an effective and stable transfection reagent.		Click on image to read more
Consistency and Reproducibility - a Reliable Transfection Reagent With NIMT®FeOfection|PURPLE you will get consistent high gene silencing with low toxicity.		Click on image to read more
The Magnetic Core can be Traced The iron oxide core gives the transfecion reagents an additional benifit: it can be traced with MRI. Transfect cells ex vivo, transplant and follow the cells with MRI.		Click on image to read more