NIMT^®FeOfection|PURPLE

NIMT^®FeOfection|PURPLE is a transfection agent specifically developed for delivery of siRNA into mammalian cell lines. With NIMT^®FeOfection|PURPLE high transfection efficiency is achieved with a minimal impact on cell viability. The transfection procedure is very easy and quick. Just add siRNA to NIMT^®FeOfection|PURPLE incubate for 20 min and then add to cells. Read out your specific knockdown 24-48 hrs post transfection.

NIMT^®FeOfection|PURPLE consists of an iron oxide core which is coated with cationic lipids to bind siRNA and promote uptake of transfection complexes into cells.

With a magnetic core NIMT^®FeOfection|PURPLE offers the possibility of combined delivery and imaging, the nanoparticles can easily be visualized with MRI.

Adherent cell lines such as HEK293, 786-O, MEF and hMSC are all successfully transfected with 90% or higher knockdown of GAPDH when transfected with a siRNA towards GAPDH using NIMT^®FeOfection|PURPLE as transfection agent.

Protocols

NIMT® FeOfection PURPLE siRNA.pdf

Application notes

siRNA Delivery into HEK293 Cells.pdf

siRNA in Human Renal Cell Carcinoma Cell Line.pdf

Knockdown of BACE-1, an Alzheimer’s Disease.pdf

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