FcDOCKER™
FcDOCKER™ binds with high affinity to all subclasses of glycosylated IgG. The protein binds selectively to the Fc part of the IgG molecule. FcDOCKER™ recognizes IgG molecules of various origin, such as human, mouse, rat, rabbit, monkey, goat and sheep. IgG is released/eluted from the FcDOCKER™ protein with sucrose.
FcDOCKER™ Features
- Elution of IgG with sucrose - no denaturation of acid sensitive IgG molecules
- Binds only to glycosylated IgG:
- useful to determine the degree of glycosylation of monoclonals
- separate non-glycosylated from glycosylated IgG
- Separate Fab from Fc fragments with high efficiency
FcDOCKER™ Compared to Other Affinity Proteins
| Species |
IgG Subclasses* |
Protein A |
Protein G |
FcDOCKER™ |
| Human |
Total IgG |
+++ |
+++ |
+++ |
| |
IgG1, IgG2, IgG4 |
+++ |
+++ |
+++ |
| |
IgG3 |
+ |
+++ |
+++ |
| |
Fab |
+ |
++ |
- |
| |
Fc |
+++ |
+++ |
++ |
| Mouse |
Total IgG |
+++ |
|
+++ |
| Rat |
Total IgG |
+ |
++ |
+++ |
| Rabbit |
Total IgG |
+++ |
+++ |
+++ |
| Goat |
Total IgG |
+ |
+++ |
+++ |
| Sheep |
Total IgG |
+ |
+++ |
+++ |
| Horse |
Total IgG |
+ |
+++ |
+++ |
| Dog |
Total IgG |
+++ |
+ |
+++ |
| Porcine |
Total IgG |
+++ |
+ |
+++ |
| *glycosylated |
|
|
|
|
 |
FcDOCKER™ was coupled to NHS activated sepharose for purification of human IgG directly from serum without further purification steps.
SDS-PAGE:
Left lane: Human serum loaded onto the column.
Right lane: Human IgG eluted with sucrose. |