NIMT® FabRICATOR
Genovis has developed a genetically modified antibody fragmentation product – NIMT® FabRICATOR - that utilizes the unique specificity of the proteolytic enzyme IdeS, which cleaves only IgG (all human subclasses, rabbit and monkey) and then only in one place. NIMT® FabRICATOR cleaves all human IgG subclasses at the same place in the hinge region creating pure F(ab’)2 fragments without any further degradation.
When you use NIMT® FabRICATOR, with its unique capability to cleave IgG exclusively at one well-defined site, the production of F(ab’)2 fragments becomes a great deal easier. There is no need to worry about reaction times, extended degradation or product integrity – your result will be the same, time after time.
With NIMT® FabRICATOR you get
- Rapid Reaction Times - Cleavage of 1mg polyclonal human IgG are finished in a matter of minutes.
- Astounding Specificity and F(ab')2 Quality - A Unique and explicit cleavage site on every IgG molecule ensures pure full F(ab')2 fragments without further degradation.
- Unmatched Yield and Reaction Consistency - With no further degradation of IgG molecules during enzymatic processing, the yield from a fragmentation reaction is phenomenal with a very high reaction to reaction consistency.
Fab and F(ab')2 antibody fragments are used in many applications or assays where the presence of the Fc region may cause problems. If antibodies are used for staining of a specific target in tissues like spleen, lymph or in peripheral blood preparations, cells with Fc receptors (macrophages, monocytes, natural killer cells and B lymphocytes) can bind to the Fc region of the antibody and cause a background staining in areas that do not contain any antigen. Fab and F(ab')2 fragments are also desirable for staining or binding to cell preparations in the presence of plasma since they can not bind to complement, which would lyse the cells. The divalency of the F(ab')2 fragment enables it to cross-link antigens and hence makes it useful for rosetting , cellular aggregation and precipitation assays.
F(ab’)2 Fragments - Pure and Simple!