FabRICATOR^®

FabRICATOR^® utilizes the unique specificity of a novel proteolytic enzyme that cleaves all human, rabbit, sheep and monkey IgG subclasses at the same place in the hinge region creating pure F(ab’)2 fragments without any further degradation. FabRICATOR^® does only cleave one subclass of mouse IgG (IgG3).  

With FabRICATOR^® You Get:

• Rapid production of F(ab')2 fragments  - Complete cleavage of IgG in minutes.
• Exquisite Specificity - Precise IgG cleavage ensures full length fragments without subsequent degradation.
• Unrivaled Yields & Consistency - Precision and efficiency results in unmatched yield and reaction consistency.

Pure F(ab')2 Fragments in Minutes

Molecular weight and marker Mark 12 (Invitrogen) IgG fraction 5 min incubation with FabRICATOR® 15 min incubation with FabRICATOR® 30 min incubation with FabRICATOR® 30 min incubation with FabRICATOR® (reduced sample) After removal of Fc fragments After removal of FabRICATOR® Molecular weight marker Mark 12

Fab and F(ab')2 antibody fragments are used in many applications or assays where the presence of the Fc region may cause problems. If antibodies are used for staining of a specific target in tissues like spleen, lymph or in peripheral blood preparations, cells with Fc receptors (macrophages, monocytes, natural killer cells and B lymphocytes) can bind to the Fc region of the antibody and cause a background staining in areas that do not contain any antigen.

Fab and F(ab')2 fragments are also desirable for staining or binding to cell preparations in the presence of plasma since they can not bind to complement, which would lyse the cells. The divalency of the F(ab')2 fragment enables it to cross-link antigens and hence makes it useful for rosetting, cellular aggregation and precipitation assays.

IgG Species Cleaved by FabRICATOR®
Human IgG	Yes	Mouse IgG1	No	Rat IgG	No
Monkey IgG	Yes	Mouse IgG2a	No*	Goat IgG	No
Rabbit IgG	Yes	Mouse IgG2b	No	Bovine IgG	No
Sheep IgG	Yes	Mouse IgG3	Yes	Porcine IgG	No
*Mouse IgG2a can be cleaved but need special instructions. Please contact Genovis support for more information.

	Product	ID	Size	Price	Quantity
	FabRICATOR®, Cleaves 2mg IgG	A0-FR1-020	2000 u	230.00 €	Add
	FabRICATOR®, Cleaves 25mg IgG	A0-FR1-250	25000 u	2,100.00 €	Add
	FabRICATOR®, Cleaves 5mg IgG	A0-FR1-050	5000 u	465.00 €	Add
	FabRICATOR®, Bulk quantity	A0-FR1-999	mg	Price on request	Add